Crime Scene Lab
Who Did It?
A female cheerleader named Caroline that went missing after a basketball game was later found dead in her boyfriend, Brian's car. Brian claimed to be innocent, but the police still arrested him. The CSI found a small drop of blood on the passengers seat from Brian's car. The camera footage from school showed Caroline last seen with Brian in his car. There was also a car following them once they left the parking lot. The police interrogated Brian and Caroline's long time friend Lauren, who was following them. They were both the last people with her and could be suspects. Evidence made it clear that Caroline was killed somewhere else, then put into Brian's car. The only way to find out who the correct suspect is, is to do the DNA blood test from each person.
Protocol
1. Pick up solutions CS, V S1, and S2 from your teacher.
2. Label 4 tubes 1, 2, 3, and 4 and add 3 uL DI water to each tube.
3. Mix solution CS by shaking the tube a couple of times. Then, using a clean tip, transfer 12 uL of the solution to tube 1.
4. Mix solution V by shaking the tube a couple of times. Then, using a clean tip, transfer 12 uL of the solution to tube 2.
5. Mix solution S1 by shaking the tube a couple of times. Then, using a clean tip, transfer 12 uL of the solution to tube 3.
6. Mix solution S2 by shaking the tube a couple of times. Then, using a clean tip, transfer 12 uL of the solution to tube 4.
2. Label 4 tubes 1, 2, 3, and 4 and add 3 uL DI water to each tube.
3. Mix solution CS by shaking the tube a couple of times. Then, using a clean tip, transfer 12 uL of the solution to tube 1.
4. Mix solution V by shaking the tube a couple of times. Then, using a clean tip, transfer 12 uL of the solution to tube 2.
5. Mix solution S1 by shaking the tube a couple of times. Then, using a clean tip, transfer 12 uL of the solution to tube 3.
6. Mix solution S2 by shaking the tube a couple of times. Then, using a clean tip, transfer 12 uL of the solution to tube 4.
Table #1
My Data
Conclusion Questions
1. On the diagram below, record the banding or color pattern in each of the lanes containing your samples. Lane 1: Crime Scene DNA
Lane 2: N/A
Lane 3: Caroline (Victim)
Lane 4: N/A
Lane 5: Brian (Suspect 1)
Lane 6: Lauren (Suspect 2)
2. According to the results from your gel, who killed the cheerleader? How did you reach this conclusion?
According to the results from my gel, Caroline's long time friend Lauren killed her. I reached this conclusion because their DNA match once the blood samples were taken.
3. After loading your gel, did any solution remain in the tubes 1, 2, 3, and/or 4? What could account for solution remaining in these tubes?
After loading my gel, blood still remained in tubes 1, 2, 3, and/or 4 because the investigators got more than enough blood information than they needed.
4. What electrical charge did the dyes that you loaded on your gel carry? How do you know?
The electrical charge that was carried through the gel was a negative electrical charge. I know this because DNA runs through electrical charges.
5. Just for fun: Why do you think the murderer killed the cheerleader?
Well, since Lauren was Caroline's "long time" friend, I think she had something against here because it seems like they aren't friends anymore, but use to be friends.
Lane 2: N/A
Lane 3: Caroline (Victim)
Lane 4: N/A
Lane 5: Brian (Suspect 1)
Lane 6: Lauren (Suspect 2)
2. According to the results from your gel, who killed the cheerleader? How did you reach this conclusion?
According to the results from my gel, Caroline's long time friend Lauren killed her. I reached this conclusion because their DNA match once the blood samples were taken.
3. After loading your gel, did any solution remain in the tubes 1, 2, 3, and/or 4? What could account for solution remaining in these tubes?
After loading my gel, blood still remained in tubes 1, 2, 3, and/or 4 because the investigators got more than enough blood information than they needed.
4. What electrical charge did the dyes that you loaded on your gel carry? How do you know?
The electrical charge that was carried through the gel was a negative electrical charge. I know this because DNA runs through electrical charges.
5. Just for fun: Why do you think the murderer killed the cheerleader?
Well, since Lauren was Caroline's "long time" friend, I think she had something against here because it seems like they aren't friends anymore, but use to be friends.